Exploring How RNC-RNA Sequencing Illuminates Translational D
When a ribosome is affixed to the polypeptide (protein) it is making, the ribosome-nascent chain complex (RNC) is the compilation of molecules that make up the ribosome. One of several methods can be used to halt the synthesis of the nascent polypeptide. RNCs are made and purified in labs to study the dynamics, biochemistry, folding,…
kikoHow to choose ANOVA?
Analysis of variance (ANOVA) is a powerful statistical method that allows researchers to compare the means of two or more groups and determine if they are significantly different. It is a popular tool for testing hypotheses in a variety of fields in biology, including ecology, genetics, physiology, and biochemistry. In this article, we will explore how…
Sequencing Depth, Coverage and Read Types for NGS
What is sequencing depth and coverage? Sequencing Depth Sequencing depth is the ratio of the total number of bases (bp) obtained by sequencing to the genome size, which is one of the indicators to evaluate the sequencing volume. There is a positive correlation between sequencing depth and genome coverage, and the error rate or false…
How to Submit Sequence Data to GenBank
Submission of sequence data to NCBI archives Next-generation sequencing, PacBio SMRT sequencing, and Nanopore sequencing, can generate numerous sequence data in a single run. Raw reads or assembled sequence need to be submitted to public sequence repository (DDBJ/ENA/GenBank – INSDC), which is required by the overwhelming majority of journals as accession numbers of theses sequence data should…
Sequencing Technologies Unveiling Plant-Microorganism
What is the microbiome? The microbiome refers to a collection of microorganisms possessing distinct physicochemical characteristics, occupying well-defined and reasonable habitats. This term encompasses not only the microorganisms themselves but also their active zones, which establish specific ecological niches. This dynamic and interactive microecosystem undergoes changes over time and scale and holds crucial importance for the functioning…
Pan-Cancer Analysis Uncovers Structural Variants as Vital…
Within the tumor genome, somatic mutations hold the ability to yield altered proteins that trigger immune responses against tumors. These mutated proteins fragment into peptides and are showcased on major histocompatibility complex (MHC) molecules. These distinct peptides, coined as “neoantigens,” play a pivotal role in anti-tumor immune activity and have emerged as promising targets for…
WGBS Reveals the Dynamic DNA Methylation Turnover in Plant
In some eukaryotes, DNA methylation occurs in the coding regions of genes and is called gene body methylation (GbM). Although the role of DNA methylation in transposon and repetitive DNA silencing has been well characterized, gene body methylation is not associated with transcriptional repression, and its biological importance is unknown. WGBS Service Whole genome…